Evaluation of Stress Biomakers (Cytokines) in Presence of Unpredictable Chronic Stress (UCS) in Sprague Dawley Rats after Treatment with the Biofield Energy Treated Novel Proprietary Test Formulation

Authors: Jana S and Trivedi MK, Branton A, Trivedi D

DOI: 10.23880/aabsc-16000163

Volume 4, Issue 2

Abstract

Unpredictable chronic stress (UCS) model was used to evaluate the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats for the estimation of serum cytokines such as TNF-Alpha, IFN-Gamma, IL-6, and IL-2, along with C-reactive protein (CRP) using ELISA assay. A test formulation was formulated including minerals (calcium, copper, iron, magnesium, selenium, and zinc), vitamins (ascorbic acid, alpha tocopherol, cholecalciferol, cyanocobalamin, and pyridoxine HCl), cannabidiol isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one part of each ingredient was defined as the untreated test formulation, while the other parts and three groups of animal received Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The cytokines estimation results showed that the level of IL-2 was reduced by 14.2% in Biofield Energy Treatment per se to the rats (G6) group as compared with the disease control group (G2). TNF-α level in serum was decreased by 11% in the G6 group as compared with the G2 group. IL-6 level in serum was decreased by 21.7%, 30.8%, 16.3%, 10.1%, and 13.4% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. However, IFN-γ level was decreased by 18.3% and 11% in the G6 and G9 groups, respectively as compared with the G4. Similarly, CRP level was significantly decreased by 25.8%, 15.2%, and 14.6% in the G5, G6, and G7 groups respectively, as compared with the G4. All-inclusive, Mr. Trivedi’s Biofield Energy Healing Treatment/Blessing per se along with other preventive treatment groups showed a significant impact on various inflammatory disorders (gout, inflammatory arthritis myositis, rheumatoid arthritis, scleroderma, etc.). Therefore, the outcomes could be slowdown the stress-related disease progression and its complications/symptoms in the preventive maintenance groups (viz. G6, G7, G8, and G9).

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