ISSN: 2639-2534
Authors: Ouedraogo S*, Ouedraogo B, Kabore B, Traore TK, Yaro A, Koala M, Yoda J, Ouedraogo S and Semde R
Introduction: Lannea microcarpa is a medicinal plant that is an alternative source of medical care for many people. It contains several compounds, some of which are present in very low concentrations. Objective: This study aims to search for a phytochemical tracer among the chemical groups in the plant for quality control. Methodology: The extracts were prepared by decoction from the trunk bark powder, freeze-dried and then fractionated successively with solvents to increase polarity. The antioxidant properties by DPPH method were studied and compared. A phytochemical screening was determined by TLC and evaluation of the contents of phytochemicals. LC-MS/MS fingerprinting of the ethyl acetate fraction was performed. Results/Discussion: The antioxidant activity of the freeze-dried aqueous extract was higher than that of the raw trunk bark powder. CCM screening indicated the presence of several compounds with three intense spots. The assays showed that phenolic compounds were in the majority. The evaluation of the fractions showed that the ethyl acetate fraction had the best content of total phenols and total flavonoids with the best antioxidant powers (IC50: 3.63 ± 0.16 μg /mL). The LC-MS/MS fingerprinting of the ethyl acetate extract revealed three major compounds detected that could serve as tracers. Conclusion: These compounds are related to the phenolic family concerning the standard myricetin, a flavonoid of the phenolic family.
Keywords: Lannea Microcarpa, Medicinal Plant, Phytochemical Tracer, Phenolic Contents, Quality Control
