Bioactivity of Crude Extracts of Secondary Metabolites of the Endophytes Phyllosticta capitalensis (Tg06) and Curvularia sp. (G6-32) against Aedes aegypti Larvae (L.1762)

Authors: Golias HC*, Polli AD, Pereira LS, Zequi JAC, Silva BP, Jorcelino TM and Pamphile JA

DOI: 10.23880/izab-16000333

Volume 4, Issue 5

Abstract

Aedes aegypti is known to transmit several arboviruses, causing economic impacts in several countries. Biolarvicides produced by endophytic fungi are a potential tool in combating mosquito proliferation. The objective of this work was to examine the potential of secondary metabolites of the endophytic fungi Phyllosticta capitalensis strain Tg06 and Curvularia sp. strain G6-32 in the control of third instar Aedes aegypti larvae. To extract the metabolites, the fermented broth was filtered with the aid of a glass and cotton funnel and then centrifuged in 50 mL conical tubes at 1400 rcf for 15 minutes. The bioassays were carried out for five days in a biological chamber at 25 ± 2°C, with a 12/12 photoperiod. The tests were performed on the following solutions: 5% dimethylsulfoxide (DMSO) and water, control solution with distilled water and a solution of crude extract of metabolites diluted in 5% DMSO and water at a concentration of 50 μg/mL. The crude extract of Phyllosticta capitalensis Tg06 caused mortality in the larvae throughout the period evaluated and Curvularia sp. G6-32 induced the interruption of larval development. The use of crude extracts of endophytic microorganisms Curvularia sp. and P. capitalensis appears to be a promising strategy for the biocontrol of Aedes aegypti and could be an alternative to the use of conventional chemical insecticides, which would result in reduction of environmental contamination and toxic effects for animals, plants, and humans.

Keywords: Bioactive Compounds; Insect Control; Larvicidal Activity; Antilarval Compounds; Antiviral Activity

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