Isolation and Identification of Methicilin Resistant Staphylcoccus aureus (MRSA) from Bovine Mastitic Milk in and around Wolaita Sodo, Southern Ethiopia

Authors: Biniam Tadesse Derib*, Biruk Tesfaye Birhanu, Tesfaye Sisay Tessema and Ashenafi Kiros Wubshet

DOI: 10.23880/oajvsr-16000136

Volume 2, Issue 3

Abstract

In recent years, there has been increased concern about antibiotic resistant strains of Staphylococcus aureus and multiple antibiotic resistant strains have started to emerge. Development of resistance has been attributed to the extensive therapeutic use of antimicrobials. A cross sectional study was conducted from November 2013 to May 2014 in and around Wolaita Sodo town, Southern Ethiopia, to isolate and identify MRSA and their resistance to different antimicrobials and also identify risk factors associated with occurrence of dairy cow mastitis. A total of 257 dairy cows were included during the study period. Total of 1010 quarters were examined to detect clinical and subclinical mastitis by physical examinations of udder and milk and California mastitis Test (CMT), respectively. Milk samples were collected from each of clinically and sub clinically mastitic non-blind quarters of the selected cows for bacterial isolation. The Staphylococcus aureus isolates were tested for anti-microbial susceptibility by disc diffusion method. The occurence of mastitis was 40.9%. Out of this, 4.66% and 36.18% were clinical and subclinical respectively. The univariate logistic regression showed that among potential risk factors considered from the farm attributes, age, milking hygiene, parity, lactation period, farm floor and previous mastitis treatment had significant (p=

Keywords: Antibmicrobials; Association; Cross Sectional; Dairy Cows; Mrsa; Quarters; Resistance, Risk Factors; Staphylococcus Aureus, Significant; Therapeutic

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