Boar Quality Semen Testing and Presence of Mycoplasma Organism

Authors:

Milovanović A, Petrović T, Apić J, Lupulović D, Barna T, Lazić G and Lazić S*

Volume 2, Issue 3

Abstract

This paper describes results of mycoplasma organism and Mycoplasma hyopneumoniae detection in semen of boars. During routine examination of semen quality in 2015 and 2016, different percent of specific changes in the spermatozoa of 23 boars were observed and these samples were subjected to mycoplasma detection. These changes were manifested as frequent distal midpiece reflex abnormalities with sporadic coiled principal piece; but booth loops were filed with fine, netlike/reticular forms (“entrapped pseudocytoplasmatic droplet”). Based on the observed morphological forms it is suspected on the presence and influence of microorganisms, primarily of Mycoplasma origin.
PCR and real time PCR molecular methods were examined in all suspected sperm samples. The presence of Mycoplasma spp was found in 15 samples, of which, Mycoplasma hyopneumoniae was found in 8 samples. In the remaining seven samples differentiation to other mycoplasma species were not carried out.
This article is indicating that genital form of mycoplasma could manifest its effect on semen quality and this may be more significant than current literature data are indicating and recognizing as problem in boars. In same time, its high incidence in suspected semen samples could be more stressed as a source of sexually transmitted infection.
Further estimation of Mycoplasma influence on boar semen quality is needed.

Keywords:

Sperm; Boars; Mycoplasma Spp; Mycoplasma Hyopneumoniae; PCR; Real Time PCR

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