Molecular Analysis of Covid-19 Patient Real Time PCR and their Medicational Clinical Trials

Authors: Muhammad Waqar Mazhar , Ujala Khan , Hira Iftikhar , Mudasara Sikandar , Abdul Manan , M Irfan and Hamna Javed

DOI: 10.23880/vij-16000284

Volume 5, Issue 3

Abstract

The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to corona viradae family and nideo virales order, envelop virus, size range 65-125nm and positive single standard RNA between 26.4 to 31.7 kb and contain7096 amino acid. There are four subtypes that have been detected these are alpha, beta, gamma and delta. The 267 covid -19 blood and nasopharyngeal samples were collected from Multan region. RNA extraction from nasopharyngeal samples and run the PCR. The blood samples use for clinical tests, Lactate dehydrogenase, serum ferritin level, D-Dimer, TG, cholesterol, thyphoidot, HDL, lymphocyte count and CRP. The 127 (47.21%) out of 267 patients were covid-19 PCR positive and showed the amplification ORF1ab, E and N gene while140 individuals were covid-19 PCR negative and not showed the amplification of ORF1ab, E and N gene. The patients with negative Covid-19 PCR, the other analysis tests such as lactate dehydrogenase, HDL, ferritin, ESR,CBP, D. Dimer, Tg, cholesterol, CRP and CT scan. The patients affected covid-19 has higher values of D-Dimer, ESR, Neutrophils, LDH, CRP and ferritin level than normal ranges. But the values of the HDL, cholesterol and lymphocytes were decreased form the normal ranges. Drugs are mentioned in table #3 that is treating for covid 19- patients. These drugs are successful for Covid -19 treatments.

Keywords: COVID-19; ESR; Delta Viruses

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