Are Bases Alkylated by Branched Alkylating Agents Removed by Nucleotide Excision Repair or Base Excision Repair?

Authors: Kotaro Sasaki, Runa Kobayashi, Takanori Nakamura, Yasuaki Kikuchi, Galbreath Hannah Elizabeth, Katsutoshi Matsumoto, Akira Hasegawa, Takayuki Saito, Kumiko Sato, Tetsuo Honma, Ayumi Yamamoto, Hirotada Arai, Yoshihiro Kadoma, Satomi Kawaguchi, Kazuyuki Furuya, Marina Kobune and Yu F Sasaki*

DOI: 10.23880/act-16000243

Volume 7, Issue 2

Abstract

We have shown that comet-positive responses by adducts that are repaired by nucleotide excision repair (NER) and base excision repair (BER) are reduced by α-amanitin (AMN) and enhanced by 2’, 3’-dideoxythymidine (ddT), respectively. Based on this finding, bases alkylated by n-alkyl groups with <5 carbons and those with >4 carbons were discussed to be removed by BER and NER, respectively. To study whether bases alkylated by branched alkyl groups can be removed by NER or BER, 4 alkyl methanesulfonates that have a branched methyl group with different branching positions [iso-propyl methanesulfonates (iPMS), 1-methyl-pentyl methanesulfonate (1MPeMS), 2-methyl-pentyl methanesulfonate (2MPeMS), and 3-methyl-pentyl methanesulfonate (3MPeMS)] were synthesized. We studied the reduction of comet-positive responses by AMN and their enhancement by ddT for those alkyl methanesulfonates with a branched methyl group.

Keywords: mation of Comet Tails, NER, BER, ddT, AMN, Bases Alkylated by Branched Alkyl Groups

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