ISSN: 2578-4811
Authors: Tugba Cuceli , Burcin Irem Abas and Ozge Cevik*
Exosomes are the smallest vesicles that can cross the blood-brain barrier and transport proteins, nucleic acids, and other cell elements to cells. The role of exosomes in drug resistance and their effects on healthy cells have been researched topics in recent years. This study investigated the effect of exosomes released from TRAIL resistance U87 glioblastoma cells on healthy L929 fibroblast cells. U87 cells were incubated with TRAIL (5ng/mL) for three weeks to develop drug resistance and exosomes were isolated with ultracentrifuge. The obtained exosomes were incubated at a concentration of 1 μg/mL-100 μg/ mL on L929 cells for 48 hours, and their cytotoxic effect was examined with MTT assay. After incubation, CXCR4 Protein and gene expression levels of CXCL-12, PAK4, and 14-3-3β were also examined. This study showed that exosomes released from TRAIL-resistant cells increase CXCR4, CXCL-12, and PAK4 levels in healthy cells and do not cause changes in 14-3-3 β oncogenic protein. While 100 μg/mL exosome affected L929 cells, no change was seen at a lower concentration. Exosomes affect the migration potential of other cells due to drug resistance. It may contribute to developing different resistance mechanisms in the long term by changing the proliferation and migration abilities of healthy cells around the tumor cell.
Keywords: Exosomes; Glioblastoma; Trail Resistance; Pak4, 14-3-3 Β; Cxcr4; Cxcl-12; Migration
