Description and Comparison of Methods for the Determination of Anti-Sars-Cov-2 Antibodies

Authors: Aznar PM , Alcaraz GP and Oldano AV

DOI: 10.23880/eij-16000249

Volume 6, Issue 4

Abstract

Introduction: The SARS-COV-2 pandemic had an impact with more than 570 million cases and more than 6.8 million deaths as recorded by the United Nations by July 2022. Within the diagnostic context, a few immunological techniques for the detection of anti-SARS-CoV-2 IgG antibodies emerged, including Chemiluminescent Microparticle Immunoassay (CMIA) and Enzyme-Linked Immunosorbent Assay (ELISA). This study aimed to compare three detection techniques that have different methodological basis. The study was observational, cross-sectional and retrospective in design. Materials and Methods: Serum samples were processed by the following methodologies: CMIA and “in-house” ELISA. Results: The methodologies were quantitatively evaluated by the correlation method, and qualitatively by assessing individual and simultaneous sensitivity and specificity. Conclusion: These methodologies were designed in a health emergency context so they do not have a gold standard; thus, different algorithms are proposed to improve both the sensitivity and specificity of the detection of SARS-CoV-2 antibodies.

Keywords: Sars-Cov-2; Anti- Sars-Cov-2 Igg Antibodies; Serologic Tests

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