ISSN: 2639-216X
Authors: Nonato LS, da Silva HCM, Silva-Gomes LC, Traldi JB, Artoni RF and Matoso DA*
Until recently, there were no reports on the methylation profile of Amazonian fish specimens employing restriction enzymes. The 18S rDNA methylation status of samples of captive tambaqui (Colossoma macropomum) exposed to the antiparasitic Trichlorfon was examined in the current study. The method performed makes use of the CCGG site's sensitivity to the presence of methylation in palindrome cytosines for the restriction enzymes HpaII and MspI. The cleavage occurs by the detection or non-detection of the methyl radical in the inner (C5mCGG) or outer (5mCCGG) cytosine, which makes it possible to compare the DNA methylation profile. When compared to the specimens examined as the control group, the results obtained revealed an increase in methylated fragments of the 18S rDNA gene at LC concentrations of 30% and 50%.
Keywords: Colossoma macropomum; Enzymatic Digestion; Pisciculture
