A Sea Star Asterias Rubens IGKappa Gene when Compared to 3 Other Sea Star Genomes from Patiria Miniata, Acanthaster Planci and Marthasterias Glacialis (Echinodermata)
The sea star IGKappa gene was cloned in 2014 by the use of primers. It was compared, in the present work, to 3 other sea star genomes: Acanthaster planci, Patiria miniata and Marthasterias glacialis sea star genomes. A high identity, from a bioinformatic point of view, was found, with these last ones, with, a significant e-value. Sea star Igkappa genes may be present in these 4 asterids which belong to the Echinoderm Phylum (Invertebrates)
Introduction
The sequence of the sea star Asterias rubens IGKappa gene was described by our team, in 2014 [1]. Since we have tried to find homologies between this gene and genes from three other Asterids: Patiria minata Acanthaster planci and Marthasterias glacialis. These Asterids belong to Echinodermata phylum.
We report, in the precedent paper, results obtained with these last ones by the use of blasts [2, 3].
Results
a) The sequence of the sea star IGKappa gene is the following [1]:
5’GGA TCC GGA GGA ATG CGTGGCAACATGGCGTCTCTATGGATGTTCTTCTT
TGTCGTGGGGATAACTTTACAACGGAGTTTGGCGATTTA- CACGTTTCGCG AGCAACCGTCGGACACTAGCGCGTTGCAGGGGAGCACAGTG- GTGCTTCAC TGCTCCGTTGAGCAGTACATAAACACCACGGCCATCGTTTG- GTGGAGCCG TGACTCGGTCATCAGCCACAACAAAGACCTGAAACTGTC- CAGTCTAAACA CCGACCAGCTCCAAAGGTACTCGATTTCAGGCGACGCATCTCG- GGGGGAA TTCAACCTTAAAATAGTGAACTTTACCGCCACAGACGCCGC- CAGTTACCG CTGTCAGATG TAA GAA TTC3’ b) BlastX original sequence: BlastX results Molecule type: DNA Query length: 357
| Description | Scientific name | Max score | Total score | Query cover | E. Value | Per. Ident | Acc Len | Accession |
|---|---|---|---|---|---|---|---|---|
| unchacterized protein LOC117296905 [Asterias rubens] | Asterias rubens | 245 | 245 | 99% | 2.00E-73 | 96,61% | 932 | XP_033635901.1 |
| unchacterized protein LOC119722929 [Patiria miniata] | Patiria miniata | 104 | 104 | 89% | 4.00E-23 | 44.25% | 951 | XP_038049262.1 |
| unchacterized protein LOC110978882 [Acanthaster planci] | Acanthaster planci | 91.3 | 91.3 | 78% | 2.00E-18 | 45.26% | 933 | XP_022089895.1 |
Table 1: BlastX original sequence.
The table above allows us to obtain the following Graphic Summary:
c) As for Alignements we observe: 97% Identities (114/118aa) with uncharacterized protein LOC117296905 [Asterias rubens] protein Reference Protein Sequence: XP_033635901.1 Reference dna Sequence: XM_033780010.1 Length : 932 aa Aligment : 14-131 d) Blastn original sequence: Blastn results Molecule type : dna Query length : 357
| Description | Scientific name | Max score | Total score | Query cover | E. Value | Per. Ident | Acc Len | Accession |
|---|---|---|---|---|---|---|---|---|
| Predicted: Asterias Rubens Uncharacterized Loc117296905 (Loc117296905), mRNA | Asterias rubens | 634 | 634 | 97% | 2.00E- 177 | 99.43% | 4538 | XM_033780010.1 |
| Asterias Rubens Genome Assembly, Chromosome: 11 | Asterias rubens | 634 | 634 | 97% | 2.00E- 177 | 99.43% | 18069988 | LR699102.1 |
| Marthasterias Glacialis Genome Assembly, Chromosome: 6 | Marthasterias glacialis | 329 | 329 | 95% | 9.00E- 86 | 84.16% | 28777708 | OU452224.1 |
Table 2: The graphic summary gives the opportunity to see the top3 Blast Hits.
As for Alignements we find a high identity nearly 100% as shown below:
99% Identities (347/349bp) with Predicted: Asterias Rubens Uncharacterized Loc117296905 (Loc117296905), mRNA Reference Sequence: XM_033780010.1 Length : 4539 Aligment : 1546-1894
Conclusion
We retain from this bioinformatic analysis, the presence of high identities between the sea star IGKappa gene and the Patiria miniata genome, Acanthaster planci one, and the Marthasterias glacialis one. We discover that sea star Asterias rubens IGKappa gene presents a strong homology to Marthasterias glacialis genome (nearly 99% identities) when compared to two other sea star genomes (Patiria miniata and Acanthaster planci ones):97% identities were found. IGKAPPA Gene may be present in at least 4 Asterids.
From another point of view, we have also described the Ophuirid IGKappa gene we discovered 1 month ago [4]: it is more evolved in terms of Immune functions than sea star IgKappa gene and Crinoïd one [5].
These genes from Echinodermata (Invertebrates) bring us a new insight in comparative Immunogenetic World.
References
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Vincent N, Osteras M, Otten P, Leclerc M (2014) A new gene in A. rubens: A sea star Ig kappa gene. Meta Gene 2: 320-322.
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Marchler-Bauer A (2017) CDD/SPARCLE: functional classification of proteins via subfamily domain architectures. Nucleic Acid Res 45(D1): 200-D203.
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Marchler-Bauer A (2011) CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acid Res 39(D): D225-D229.
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Leclerc M (2021) Biosynthesis « De Novo » of the Ophuirid Ophiocomina Nigra Igkappa Gene. J Clin Class Immunol 1(1): 1-4.
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Leclerc M (2021) Comparisons Between 3 Invertebrates Igkappa Genes: From the less Evolved to the Most One. Bioinformatic Data. J Virol Viral Dis 1(1).
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