Histopathological Investigation of Prawn Penaeus monodon in the Improved Extensive Culture Ponds of Nellore Region, Andhra Pradesh, India

Authors: Sivachandrabose K and Mohan S*

DOI: 10.23880/izab-16000482

Volume 6, Issue 4

Abstract

Present work was aimed to see the quality of the prawn Penaeus monodon under improved extensive aquaculture system collected from Muthukur area of Andhra Pradesh located Pradesh located at 14.29 19 latitude and 80.10383 longitude. To assess the quality of the prawn, prawns were surveyed in the improved extensive pond and collected for histopathological investigation by adopting double staining methods (hematoxylin and eosin). Results showed that WSSV (White Spot Syndrome Virus) was characterised by prominent eosinophilic to pale basophilic intra nuclear inclusion bodies in hypertrophied nuclei of most commonly in the epithelial cells and connective tissue cells of the above target tissues. Monodon baculovirus (MBV) was characterised by intensive stained or multiple spherical or rounded hypertrophied intra nuclear inclusion bodies ranging from 0.1 m to 18 m in the epithelial cells of hepatopancreas. This kind of necrotic behaviour was also found along with acidophilic intra nuclear inclusion in hypertrophic nuclei with marginated chromatin. MBV infected shrimps showed generalised signs of disease such as lethargy, anorexia, poor feeding, dark colouration and reduced growth rate. Intensive stained basophilic hypertrophic rounded intranuclear inclusions symptoms were also found due to those disease both in eye and gill tissues. It is predicted that the prawn collected from the pond showed the symptoms of both viral and bacterial infection.

Keywords: Present work was aimed to see the quality of the prawn Penaeus monodon under improved extensive aquaculture system collected from Muthukur area of Andhra Pradesh located Pradesh located at 14.29 19 latitude and 80.10383 longitude. To assess the quality of the prawn, prawns were surveyed in the improved extensive pond and collected for histopathological investigation by adopting double staining methods (hematoxylin and eosin). Results showed that WSSV (White Spot Syndrome Virus) was characterised by prominent eosinophilic to pale basophilic intra nuclear inclusion bodies in hypertrophied nuclei of most commonly in the epithelial cells and connective tissue cells of the above target tissues. Monodon baculovirus (MBV) was characterised by intensive stained or multiple spherical or rounded hypertrophied intra nuclear inclusion bodies ranging from 0.1 m to 18 m in the epithelial cells of hepatopancreas. This kind of necrotic behaviour was also found along with acidophilic intra nuclear inclusion in hypertrophic nuclei with marginated chromatin. MBV infected shrimps showed generalised signs of disease such as lethargy, anorexia, poor feeding, dark colouration and reduced growth rate. Intensive stained basophilic hypertrophic rounded intranuclear inclusions symptoms were also found due to those disease both in eye and gill tissues. It is predicted that the prawn collected from the pond showed the symptoms of both viral and bacterial infection.

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