ISSN: 2574-7800
Authors: Turkmenoglu MF, Pektas SD, Bilgic AD, Ozdemir C* and Edgunlu TG
Background: Psoriasis is an inflammatory skin disease. Ghrelin protein exhibits an anti-inflammatory effect by inhibiting the TNF-α/NF-κB pathway in psoriasis. The aim of this study was to investigate whether the GHRL gene rs27647 and rs34911341 polymorphisms are associated with susceptibility to generalized Early Onset Psoriasis (EOP). Material and Methods: The study group consisted of 80 EOP patients and 70 unrelated healthy controls. GHRL polymorphisms were determined using the polymerase chain reaction (PCR)-restriction fragment length polymorphism method. In addition, we determined other genes with which the GHRL gene interacts, and the effect on protein function and protein stability for rs34911341. We used web-based tools for all in silico analyses. Results: Our results have shown GHRL gene rs34911341 polymorphism GA genotype is associated with an increased risk of EOP (p=0.000). Also, there was a significant difference between the two groups with regard to the allele frequency of rs34911341 polymorphism (p=0.000). We have shown the prediction of Gene-gene interaction that GHRL is co-expressed with 18 genes, physical interaction with 16 genes and co-localization with 9 genes. We determined deleterious of rs34911341 in 7 tools and revealed that rs34911341 decreased protein stability significantly. Conclusion: The GHRL rs34911341 polymorphism genotype and allele frequencies of the EOP patients differ significantly from those of healthy controls. We obtained in silico analysis results that support molecular data for rs34 polymorphism in the GHRL gene. This may be responsible for explaining chronic inflammation in the pathogenesis of EOP.
Keywords: Early Onset Psoriasis; GHRL; Polymorphism
