Objective: to determine whether concentration of beta HCG in cervico-vaginal secretion could be used to predict preterm delivery in women presenting with signs and symptoms suggestive of preterm labour Design: Hospital based prospective study. Method: This study was conducted in Central Women’s Hospital, Yangon, Myanmar. It included a total of 148 pregnant women presenting with signs and symptoms suggestive of preterm labour between 24 to 34 weeks of gestation. Exclusion criteria included those with foetal congenital anomalies, vaginal bleeding, foetal growth restriction, foetal distress, multiple gestation, cervical dilatation ≥3 cm or preterm rupture of membrane. Eligible pregnant women were offered for participation in the study. Concentration of beta HCG in the cervico-vaginal secretion was detected by qualitative beta HCG one step pregnancy test strip. The result obtained was kept confidential and all the patients were managed as per protocol for suspected preterm labour. Follow up of the outcome, either term or preterm delivery, was recorded. Data collection was done by completing the proforma and data analysis was done by statistical software, SPSS version 16. Results: The majority of the patients in this study were 27.73(6.24) years. Nulliparity group constituted 56.1% and history of preterm delivery was present in 10 patients among 65 multiparous patient. Mean gestational age at the time of presentation of the study group was 31.39 ± 2.7 weeks. Beta HCG test was positive in 53.4% (79 out of 148) of the patients. Among them, 73 patients were delivered before 37 weeks. The prevalence of preterm delivery in the study group was 68.2% (101 out of 148) and mean gestational age at delivery was 34.81 (3.43) weeks. Mean test to delivery interval was 23.4 (25.69) days. Predictive values for preterm delivery of cervico-vaginal beta HCG were sensitivity of 72.3%, specificity of 87.2%, positive predictive value of 92.4% and negative predictive value of 59.4% respectively. Likelihood ratio for positive and negative beta HCG test were 5.66 and 0.31 respectively. There was statistically significant association between positive beta HCG test and preterm delivery (χ2 = 61.045, p<0.001). Conclusion: Qualitative assessment of beta HCG in cervico-vaginal secretion could be a useful predictor of preterm delivery in women presenting with signs and symptoms suggestive of preterm labour.