Expression and Purification of the Recombinant OmpL1 Protein for Potential Vaccine Production against Leptospirosis

Authors: Nguyen Tuan Hung, Nguyen Van Huy, Nguyen Duc Hieu, Dang Thi Quynh, Vo Thi Bich Thuy* and Nghiem Ngoc Minh

DOI: 10.23880/izab-16000252

Volume 3, Issue 6

Abstract

Leptospirosis is a popular zoonosis caused by pathogenic strains of the Leptospira. OmpL1 is the outer membrane protein present in most pathogenic Leptospira strains and consider an important factor in the immunogenicity response. We describe here a simple procedure for generating recombinant OmpL1 protein resistant to Leptospira. OmpL1102-316 recombinant was cloned on Escherichia coli strain DH10b and expressed in strain BL21. The purification has performed by an affinity-linked Ni- Sepharose column with His-tag attached to this protein. The recombinant protein OmpL1 induced immunological memory in laboratory rats, suggesting a potential recombinant vaccine production for using in against Leptospirosis in animals.

Keywords: rLipL21; Recombinant Protein Vaccine; Leptospira interrogans; Vietnam

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