Ganoderma lucidum Modulates Neuronal Distorted Cytoskeletal Proteomics and Protein-Protein Interaction in Alzheimer’s disease Model Animals

Authors: Rahman MA*, Hossain S, Abdullah N and Aminudin N

DOI: 10.23880/nnoaj-16000155

Volume 6, Issue 1

Abstract

Background: Alzheimer’s disease (AD) is the leading neurodegenerative disorder affecting memory, learning and behavior. Altered expression of proteins involved in neuronal structure and function is a recent observation of AD pathogenesis. Modulation of altered protein expression seems promising in AD therapeutics. In the present experiment, AD ameliorating effect of medicinal mushroom Ganoderma lucidum had been evaluated through its effect on neuronal cytoskeletal structure and function related protein expression pattern in AD model rats. Methods: Wistar male rats (120 ± 5gm) were divided into three groups: control (C), AD (A) and G. lucidum hot water extract (HWE) fed AD (AE), each group containing 15 rats. AD model rats were prepared by infusing Aβ1-42 (ab120959, abcam, USA) into the cerebral ventricles. Protein extraction from the brain samples was performed following homogenization of the hippocampus (50 mg ) with lysis buffer (1ml) using a homogenizer (Polytron PT 1200, Kinematica). Protein separation through SDS-PAGE and protein quantification through LC-chip MS/MS Q-TOF had been performed for label-free relative quantification. For statistical analyses, the data were exported to the Mass Profiler Professional (MPP) software and ANOVA (P

Keywords: : Alzheimer’s Disease; Neuroproteomics; Iingenuity Pathway Analysis; Protein-protein interaction; STRING

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