In Vitro Anti-Cancer, Anti Metabolic and Anti Proliferative Activity of Phytolacca decandra 6CH and 200CH in Breast Cancer Cell Lines

Authors: Raja KMU* , Amma CRKK , Vargheese WV and Gopukumar ST

DOI: 10.23880/nnoa-16000220

Volume 7, Issue 2

Abstract

Phytolacca decandra is specific medicine used for treatment of breast cancer although many literatures available regarding its utility. Evidence based action is needed for its action on breast cancer therefore we aimed to assess In vitro cytotoxicity effects of Phytolacca decandra 6CH, 200CH in Breast cancer cell lines. cytotoxicity and antiproliferative effects in breast cancer lines exposed to Phytolacca decandra 6CH, 200CH as revealed by MTT assay, metabolic activity was tested using SRB assay. Ability to inhibit growth of cancer cells was evaluated by colony forming assay. Phytolacca decandra 6CH and 200CH has exhibited antiproliferative effect and significant cytotoxicity as revealed by MTT assay. Initially dose was optimized for each sample and once the IC50 value was determined we checked its cytotoxicity using MTT assay. The metabolic activity of the Phytolacca decandra 6CH and 200CH was tested using SRB assay. This too revealed that the Phytolacca decandra 6CH and 200CH were able to reduce the metabolic activity of then cancer cells. Colony forming assay was done to check the ability of the Phytolacca decandra 6CH and 200CH to inhibit the growth of cancer cells. The results revealed that the Phytolacca decandra 6CH and 200CH drastically decreased the multiplication of the MCF 7 cancer cells and thus proving its anticancer activity. This result shows evidence-based Homoeopathy medicine Phytolacca decandra 6CH and 200CH has anticancer activity.

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