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NNOA Research Article May 25, 2018
Efficiencies of the Cryoprotectants N-Methylacetamide, N-Methylformamide and Dimethyl Sulfoxide, and the Cell Protectants Trehalose and Hydroxyethyl Starch in Cryopreservation of Swine Sperm
Osuga T1*, Hasegawa T2, Aoki M1, Fujie M3, Nakane T2 and Asai T4

Polar molecules protect against freeze-induced damage by interfering with the polarised water arrangement during ice nucleation inside cells. Insufficient protection from freeze-in…

Freeze-induced damage Cryopreservative-induced cytotoxicity Cell permeation
NNOA Research Article Mar 5, 2018
Methylation of the Cryopreservative N-Methylacetamide into N,N-Dimethylacetamide in the Living Body
Osuga T1*, Aoki T2, Tsuda T3, Sugiyama H4, Tamura Y5 and Asai T6

The polar molecule dimethyl sulfoxide (DMSO) protects against freeze-induced damage by interfering with the polarised water arrangement during ice nucleation inside both nucleated …

Freezing-Induced Damage Cryopreservative-Induced Cytotoxicity Hepatotoxicity
NNOA Research Article Apr 18, 2017
N-Methylacetamide for Cryopreservation - An Alternative to Dimethyl Sulfoxide
Osuga T1*, Shimizu N2, Takanashi M3, Tamura Y4, Makino S5 and Asai T6*

Cell death due to cryopreservation is caused by insufficient protection from freezing-induced damage and cytotoxicity of the cryopreservation agent, acting both before freezing and…

Hematopoietic Stem Cell Cryoprotectant Freezing-Induced Damage
IZAB Research Article Jun 24, 2022
Fecal Cortisol Dynamics of Captive Giraffes in Zoos in Northern Japan
Satoh Y, Yoshihara K, Nakamura S, Amao A, Sasaki W, Nakanowataru Y, Sakurama M and Kooriyama T*

Animals in zoos are kept under environmental conditions differing from those of their original habitat. Recently, zoos have been attempting to reduce stress by introducing environ…

Captive Fecal Cortisol Giraffe
JOBD Research Article May 12, 2022
Reliability of the Posterior Condylar Axis as an Alternative Reference for Femoral Rotation in Japanese Patients with Varus Knee Osteoarthritis Undergoing Total Knee Arthroplasty
Yosuke Hattori*, Nobuyuki Asai, Shotaro Mori, Ken Ikuta, Yusuke Kazama, Yusuke Iesaki, Shimpei Takahashi, Atsushi Kaneko and Tomotaro Sato

Background: This study aimed to assess the reliability of the posterior condylar axis (PCA), the most widely used alternative reference for the surgical transepicondylar axis (sTEA…

Posterior Condylar Axis Surgical Transepicondylar axis Total Knee Arthroplasty
NNOA Research Article Jun 25, 2018
Preparation & Characterization of Bosentan Loaded Ethyl cellulose Nanoparticles by Solvent Evaporation Technique
Tabassum A and Krishnasailaja A*

The aim of this study was to prepare Bosentan loaded nanoparticles by Solvent Evaporation (SE) technique. Bosentan belongs to a class of drugs known as endothelin receptor antagoni…

Nanoparticles Solvent Evaporation Bosentan Monohydrate
GHIJ Review Article Sep 22, 2016
Pharmacotherapy of Giardiasais: Past, Present & Future
Gupte S* and Gupte N

Objective: In view of the scattered information about pharmacotherapy of the common protozoal infestation in the English medical literature, this update aims at providing a state-o…

Alkbendazole Giardiasis Firazolidine