The Asterias Rubens Sea Star Igkappa Gene When Compared to Marthasterias Glacialis Sea Star Genome(Echinodermata)
The sea star IGKappa gene was cloned in 2014 by the use of primers. It was compared in the present work to Marthasterias glacialis sea star genome. A high identity was found with this last one.
Introduction
The sequence of the sea star Asterias rubens IGKappa gene was described by our team, in 2014 [1]. Since we have tried to find homologies between this gene and genes from another Asterid: Marthasterias glacialis We report, in the 5’GGA TCC GGA GGA ATG CGTGGCAACATGGCGTCTCTATGGATGTTCTTCTT TGTCGTGGGGATAACTTTACAACGGAGTTTGGCGATTTACACGTTTCGCG AGCAACCGTCGGACACTAGCGCGTTGCAGGGGAGCACAGTGGTGCTTCAC TGCTCCGTTGAGCAGTACATAAACACCACGGCCATCGTTTGGTGGAGCCG TGACTCGGTCATCAGCCACAACAAAGACCTGAAACTGTCCAGTCTAAACA CCGACCAGCTCCAAAGGTACTCGATTTCAGGCGACGCATCTCGGGGGGAA TTCAACCTTAAAATAGTGAACTTTACCGCCACAGACGCCGCCAGTTACCG CTGTCAGATG TAA GAA TTC3’
Results
precedent paper, results obtained with these last ones by the use of blasts against human genes [2, 3].
The sequence of the sea star IGKappa gene is the following [1]:
| Description | Scientific name | Max score | Total score | Query cover | E. Value | Per. Ident | Acc Len | Accession |
|---|---|---|---|---|---|---|---|---|
| Predicted: Asterias Rubens Uncharacterized Loc117296905 (Loc117296905), mRNA | Asterias rubens | 634 | 634 | 97% | 2.00E-177 | 99.43% | 4538 | XM_033780010.1 |
| Asterias Rubens Genome Assembly, Chromosome: 11 | Asterias rubens | 634 | 634 | 97% | 2.00E-177 | 99.43% | 18069988 | LR699102.1 |
| Marthasterias Glacialis Genome Assembly, Chromosome: 6 | Marthasterias glacialis | 329 | 329 | 95% | 9.00E-86 | 84.16% | 28777708 | OU452224.1 |
Table 1: ** Blastn Original Sequence, Blastn results.
Molecule type: DNA Query length: 357 Table 1: Blastn Original Sequence, Blastn results.
The graphic summary gives the opportunity to see the top3 Blast Hits:
As for Alignements we find a high identity nearly 100% as shown below:
99% Identities (347/349bp) with Predicted: Asterias Rubens Uncharacterized Loc117296905 (Loc117296905), mRNA Reference Sequence: XM_033780010.1 Length: 4539 Aligment: 1546-1894
Conclusion
We retain from this bioinformatic analysis, a high identity between the Asterias rubens sea star IGKappa gene with the sea star Marthasterias glacialis genome. Those genes, nevertheless, seem less evolved that the Ophuirid IGKappa gene we discovered 1 month ago [4] in terms of Immune functions.Sea stars belong to Asterid class. They are Echinodermasta like Ophuirids.
References
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Vincent N (2014) A new gene in A. rubens: A sea star Ig kappa gene. Meta Gene 2: 320-322.
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Marchler Bauer A, Bo Y, Han L, He J, Lu S, et al. (2017) CDD/SPARCLE: functional classification of proteins via subfamily domain architectures Nucleic Acid Res 45(D): 200-203.
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Marchler-Bauer A (2011) CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acid Res 39(D): 225-229.
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Leclerc M (2021) Biosynthesis «De Novo» of the Ophuirid Ophiocomina Nigra Igkappa Gene. J Clin Class Immunol 1(1).
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