On the Treatment of SARS-COV-2 Including Omicron Type

Introduction

The most recent common ancestor (MRCA) of all coronaviruses has been placed at around 8000 BCE [1]. The MRCAs of the Alpha-coronavirus line has been placed at about 2400 BCE, the Beta-coronavirus line at 3300 BCE, the Gamma-coronavirus line at 2800 BCE, and the Delta coronavirus line at about 3000 BCE. It appears that bats and birds, as warm-blooded flying vertebrates, are ideal hosts for the coronavirus gene source (with bats for Alpha-coronavirus and Beta coronavirus, and birds for Gamma-coronavirus and Delta-coronavirus) to fuel coronavirus Evolution and dissemination [2]. Bovine coronavirus and canine respiratory coronaviruses diverged from a common ancestor in 1951 [3]. Bovine coronavirus and human coronavirus OC43 diverged around the 1890s. Bovine coronavirus diverged from the equine coronavirus species at the end of the 18th century [4]. The MRCA of human coronavirus OC43 has been dated to the 1950s [5]. MERS-CoV, although related to several bat coronavirus species, appears to have diverged from these several centuries ago [6]. The human coronavirus NL63 and a bat coronavirus shared an MRCA 563–822 years ago [7]. The most closely related bat coronavirus and SARS-CoV-2 diverged in 1986 [8]. A path of evolution of the SARS virus and keen relationship with bats has been proposed. The coronaviruses have been coevolved with bats for a long time and the ancestors of SARS-CoV first infected the species of the genus Hipposideridae, subsequently spread to species of the Rhinolophidae and then to civets, and finally to humans [9, 10]. Alpaca coronavirus and human coronavirus 229E diverged before 1960 [11]. The information above demonstrates that even 8000 years ago and so on, all the kinds of COVID-19 existed, Alpha, Beta, Gamma and even Delta kinds of this disease existed and it is not a novel or new infectious disease we are fighting against. Drift and also Reassortment plays vital role in mutations and adaptation of COVID-19 specially Omicron which is nowadays contaminated high amounts of people and will be present in the future.

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Figure 2: Illustration of the locations of the Omicron mutations in the spike protein, top Left picture and right picture, showing amino acid substitutions which have shown in color yellow, deletions (red), and insertions (green). In this trimeric structure, two monomers (gray and light blue) have their receptor-binding domains in the down conformation while one (dark blue) is in the up or open conformation. Mutation data from WHOn structure from PDB: 6VYB​ [12, 13].

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Materials and Methods

Since the Omicron virus has strongly high rate of mutation compared with other kinds of Coronaviruses and therefore; cause severe symptoms of inflammation in the patients including lungs as we observed in 2 of our patients with high age. In our research, all the patients were under unique control and it was a controlled study. They received HBO2T with 2% Ozone mixture with Oxygen in special cube. Also they had an injection of liquid ozone which dramatically declines the inflammation in all tissues. They had under strict diet of special Ketogenic diet to maintain their mitochondria to remain high metabolism to hamper the fatigue and tiredness. The had also gain supplements necessary for the disease. The Ketogenic diet we used was 60% fat and 40% high fiber carbohydrates. They had Amino Acid 10% Serum once a day in the morning not to destroy the and diminish the muscles in their body and also maintain their dosage of amino acid since they did not have appetite to eat regularly. Plus, they had Dextrose/salt 9% serum with B-Complex, special Neurobion and Vitamin C injected into their serum once a day in the evening. Their supplements included 1200 mg of NAC, 30 mg Zinc Oxide, Vitamin b6 40 mg, Vitamin B1 100mg every night after their meal. We gave those 500mg vitamin c every two hours to maintain their blood of vitamin C levels to decrease the inflammation as well. Medical ozone is produced in varying concentrations. The quantity of ozone in comparison with the quantity of oxygen in the gas stream is called percent concentration. It is measured in micro grams (ug) of ozone per milliliter (or cc) of the mixture. A liter of oxygen weights 1.4 grams. Therefore; 0.5% x 1.4 gm = 7 ug/cc, 1.0% x 1.4 gm = 14 ug/cc, 1.5 x 1.4 gm = 21 ug/cc, 2.0% x 1.4 gm = 28 ug/cc, 2.5% x 1.4 gm = 35 ug/cc, 3.0% x 1.4 gm = 42 ug/cc, 3.5% x 1.4 gm = 49 ug/cc, 4.0% x 1.4 gm = 56 ug/cc, 4.5% x 1.4gm = 63 ug/cc, 5.0% x 1.4 gm = 70 ug/ cc [14]. 5% or 70 ug/cc is considered to be the upper limit of the concentration for the internal use of medical ozone. The medical internal ozone therapy in Omicron or Covid-19 treatment. We used the average concentration means 21 ug/cc. This method of Ozone Therapy with liquid ozone injection, higher the blood flow through the body, decrease inflammation in the whole body, decrease inflammation in lungs dramatically and the most important which we also intrigued by was that this method stopped the virus activation in the tissues and the body.

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Results

All the patients of any age showed in the table got treatment in three days. After 6 days all the side effects of Omicron virus diminished. They regain their strength and their weaknesses, muscle pain, tiredness, joint pain and bone pain went away. We call this novel protocol: Sorush Covid-19 Treatment Protocol. Although it needs some more research in this field and protocol, but it is a huge breakthrough in Covid control and treatment.

Acknowledgement

We would like to thank the US Department of Defense, American Beirut University, Sen. Mark R. Warner, Sen. Kaine and Representative Ben Cline for their encouragement in our research. Also we would like to specifically thank Professor Stephanie Seneff PhD from Massachusetts Institute of Technology and Professor Thomas N Seyfried who always encouraged and motivated me.